Kanokorn WechakornSamran PrabpaiKanoknetr SuksenPhongthon KanjanasiriratYongyut PewkliangSuparerk BorwornpinyoPalangpon KongsaereeMahidol University2019-08-232019-08-232018-02-01Luminescence. Vol.33, No.1 (2018), 64-7015227243152272352-s2.0-85024100001https://repository.li.mahidol.ac.th/handle/20.500.14594/45250Copyright © 2017 John Wiley & Sons, Ltd. A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1002/bio.3373