Sara StincaMaria AnderssonSandra WeibelIsabelle Herter-AeberliRalph FingerhutSueppong GowachirapantSonja Y. HessNidhi JaiswalTomislav JukićZvonko KusicNgoako Solomon MabapaAshwini Kumar NepalTeofilo O.L. San LuisJia Qing ZhenMichael Bruce ZimmermannETH ZurichKinderspital ZürichMahidol UniversityUniversity of California, DavisSt. John's National Academy Of Health Sciences IndiaUniversity of Zagreb School of MedicineUniversity of Venda for Science and TechnologyB P Koirala Institute of Health SciencesIodine Global Network (IGN)Shanxi Institute for Endemic Disease Prevention and Treatment2018-12-212019-03-142018-12-212019-03-142017-01-01Journal of Clinical Endocrinology and Metabolism. Vol.102, No.1 (2017), 23-32194571970021972X2-s2.0-85010617560https://repository.li.mahidol.ac.th/handle/20.500.14594/42100© 2017 by the Endocrine Society. Context: Thyroglobulin (Tg) could be a sensitive biomarker of iodine nutrition in pregnant women (PW). A dried blood spot (DBS) assay would simplify collection and transport in field studies. Objectives: Our aims were to (1) establish and test a reference range for DBS-Tg in PW; (2) determine whether co-measurement of Tg antibodies (Abs) is necessary to define population iodine status. Design, Setting, and Participants: Standardized cross-sectional studies of 3870 PW from 11 countries. For the DBS-Tg reference range, we included TgAb-negative PW (n = 599) from 3 countries with sufficient iodine intake. Main Outcome Measures: We measured the urinary iodine concentration and DBS thyroidstimulating hormone, total thyroxin, Tg, and TgAb. Results: In the reference population, the median DBS-Tg was 9.2 μg/L (95% confidence interval, 8.7 to 9.8 mg/L) and was not significantly different among trimesters. The reference range was 0.3 to 43.5 mg/L. Over a range of iodine intake, the Tg concentrations were U-shaped. Within countries, the median DBS-Tg and the presence of elevated DBS-Tg did not differ significantly between all PW and PW who were TgAb-negative. Conclusions: A median DBS-Tg of ∼10 mg/L with ,3% of values ≥44 μg/L indicated population iodine sufficiency. Concurrent measurement of TgAb did not appear necessary to assess the population iodine status.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1210/jc.2016-2829