Buddhirongawatr R.Tantawiwattananon N.Sangloung C.Sukthana Y.Ruangrat BuddhirongawatrNitipan TantawiwattananonCharoonluk SangloungYaowalark Sukthanaรวงรัตน์ พุทธิรงควัตรเยาวลักษณ์ สุขธนะนิธิพันธ์ ตัณฑะวิวัฒนานนท์Mahidol University. International College. Planning, Research and Development Department.2014-10-212018-02-272014-10-212018-02-272014-10-212006Southeast Asian Journal Tropical Medicine and Public Health. Vol.37 (Suppl. 3), (2006), 15-17https://repository.li.mahidol.ac.th/handle/20.500.14594/9887Toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. Live tachyzoites derived from serial passage in HeLa culture were used in the Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (Prion nailurus viverrina), one leopard (Panthera pardus), two flatheaded cats (Prion nailurus planiceps), 6 tigers (Panthera tigris), two leopard cats (Felis bengalensis), two clouded leopards (Felis nebulosa), 3 pumas (Puma concolor), and 4 jungle cats (Felis chaus). Antibodies to Toxoplasma gondii were founded in 9 of 21 felids (42.8%). This study revealed that cell culture-derived tachyzoites can be used successfully as a source of live organism in a gold standard Sabin-Feldman dye test, which is simpler, cheaper and less ethically sensitive than in vivo inoculation.engMahidol UniversityToxoplasma gondii antibodyEpidemiologyWild lifeDetection of toxoplasma gondii in captive wild felids.Article