Ruchuon YokchomSomsak LaiwejpithayaWeerakanya ManeeprakornSatita TapaneeyakornJundee RabablertTararaj DharakulSilpakorn UniversityThailand National Nanotechnology CenterFaculty of Medicine, Siriraj Hospital, Mahidol University2019-08-232019-08-232018-04-01Nanomedicine: Nanotechnology, Biology, and Medicine. Vol.14, No.3 (2018), 1051-105815499642154996342-s2.0-85042764349https://repository.li.mahidol.ac.th/handle/20.500.14594/45204© 2018 Elsevier Inc. The aim of this study was to develop a paper-based immunosensor for cervical cancer screening, with signal amplification by multifunctionalized gold nanoparticles (AuNPs). The AuNPs were functionalized with a highly specific antibody to the p16 INK4a cancer biomarker. The signal was amplified using a combination of the peroxidase activity of horseradish peroxidase (HRP) enzyme-antibody conjugate and the peroxidase-like activity of the AuNPs. The immune complex of p16 INK4a protein and multifunctionalized AuNPs was deposited on the nitrocellulose membrane, and a positive result was generated by catalytic oxidation of peroxidase enzyme substrate 3,3’,5,5’-Tetramethylbenzidine (TMB). The entire reaction occurred on the membrane within 30 min. Evaluation in clinical samples revealed 85.2% accuracy with a kappa coefficient of 0.69. This proof of concept study demonstrates the successful development of a highly accurate, paper-based immunosensor that is easy to interpret using the naked eye and that is suitable for cervical cancer screening in low-resource settings.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringEngineeringMaterials ScienceMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/j.nano.2018.01.016