Koirala P.Bhattarai P.Sriprablom J.Zhang R.Nirmal S.Nirmal N.Mahidol University2025-01-232025-01-232025-01-01International Journal of Biological Macromolecules Vol.285 (2025)01418130https://repository.li.mahidol.ac.th/handle/20.500.14594/102743Chitosan is a deacylated derivative of chitin, which is a naturally occurring polysaccharide found in the shells of crustaceans. Chitosan's biocompatibility, physicochemical and mechanical properties qualify it as an excellent candidate for biomedical and pharmaceutical applications. Furthermore, the nanoengineering of chitosan enhances its functional and desirable properties for various applications. Additional functionalization of nano-chitosan is possible using various crosslinkers via chemical modification of hydroxyl or amino groups chitosan. This advanced functionalized nano-chitosan enables drug stability, site-specific delivery, controlled release, and sustainable pharmacodynamic properties. It is also used as a regenerative medicine for wound healing, bone and dental repair, biosensing and disease detection, tissue engineering, etc. Therefore, this review discusses the functionalization of nano-chitosan. A brief discussion is provided on the applications of nano-chitosan in the pharmaceutical industry such as drug carriers, targeted delivery, and controlled release, as well as in the biomedical industry, including wound healing, bone, and dental repair. Additionally, the disease detection using nano-chitosan has been investigated. Lastly, future perspectives and concluding remarks are presented.Biochemistry, Genetics and Molecular BiologyReviewSCOPUS10.1016/j.ijbiomac.2024.1383242-s2.0-8521102513218790003