Banthit ChetsawangJirapa ChetsawangPiyarat GovitrapongMahidol University2018-09-242018-09-242010-10-18Journal of Biomedicine and Biotechnology. Vol.2010, (2010)11107251111072432-s2.0-77957838992https://repository.li.mahidol.ac.th/handle/20.500.14594/28616It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxide-induced toxicity in neuronal cells. Copyright 2010 Jirapa Chetsawang et al.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEnvironmental ScienceArticleSCOPUS10.1155/2010/803815