Ihn Kyung JangAbby TylerChris LymanJohn C. RekEmmanuel ArinaitweHarriet AdramaMaxwell MurphyMallika ImwongStephane ProuxWarat HaohankhunnathamRebeccar BarneyAndrew RashidMichael KalnokyMaria KahnAllison GoldenFrançois NostenFrançois NostenBryan GreenhouseDionicia GamboaGonzalo J. DomingoInfectious Diseases Research CollaborationUniversidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von HumboldtShoklo Malaria Research UnitPATH SeattleUniversity of California, San FranciscoMahidol UniversityNuffield Department of MedicineQuansys Biosciences2020-08-252020-08-252020-06-01American Journal of Tropical Medicine and Hygiene. Vol.102, No.6 (2020), 1366-136914761645000296372-s2.0-85086051620https://repository.li.mahidol.ac.th/handle/20.500.14594/57975© 2020 by The American Society of Tropical Medicine and Hygiene. Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generationRDTdevelopment and screen asymptomatic malaria,we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparumspecific lactate dehydrogenase (Pf LDH), Plasmodium vivax-specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.19-0763