Sarawut JitrapakdeeAnchalee TassanakajonVichai BoonsaengSuwit PiankijagumSakol PanyimMahidol UniversityChulalongkorn University2018-07-042018-07-041995-01-01Molecular and Cellular Probes. Vol.9, No.6 (1995), 375-382089085082-s2.0-0029587426https://repository.li.mahidol.ac.th/handle/20.500.14594/17253A polymerase chain reaction (PCR) method was developed for detection of salmonella in food. A set of PCR primers was designed to amplify a 199 bp salmonella-specific DNA fragment derived from a repetitive DNA of Salmonella Weltevreden. The assay detected all 52 most prevalent serovars found in contaminated food in Thailand and no cross-reaction was observed with other non-salmonella organisms. The limit of detection was 1 fg of purified target DNA or five bacteria from pure culture. The detection of artificially contaminated food performed following a 6 h enrichment step was three bacteria per gram and the result was obtained within 4 h. © 1995 Academic Press Limited.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1006/mcpr.1995.0059