Khamwut A.Nimnual J.Chomta N.Nimsamer P.Mayuramart O.Kaewsapsak P.Pasittungkul S.Poovorawan Y.Payungporn S.Mahidol University2025-05-262025-05-262025-01-01Experimental Biology and Medicine Vol.250 (2025)15353702https://repository.li.mahidol.ac.th/handle/20.500.14594/110375Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.3389/ebm.2025.103872-s2.0-10500529190915353699