Porntip H. LolekhaPornpen NitipaichitMahidol University2018-08-102018-08-101992-01-01Journal of Clinical Laboratory Analysis. Vol.6, No.5 (1992), 245-25610982825088780132-s2.0-0026669984https://repository.li.mahidol.ac.th/handle/20.500.14594/22272Beckman aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol, triglycerides, urea, and uric acid liquid Reagents for Synchron CX 4/5 (48, 48, 25, 60, 26, and 30 cents US/test, respectively) are expensive. We have established our own methods for serum AST, ALT, cholesterol, triglycerides, urea, and uric acid (6, 6, 5, 12, 13, and 6 cents US/test, respectively) using Ames Sera‐Pak reagents. Linearity of our AST, ALT, cholesterol, triglycerides, urea, and uric acid methods were either similar to or higher than the Beckman mehtods. The within run and day‐to‐day run precisions were acceptable. Recovery of our AST, ALT, cholesterol, triglycerides, urea, and uric acid wee excellent. Our results for AST, ALT, cholesterol, triglycerides, urea, and uric acid correlated well with the Beckman results. Bilirubin (340.8 μmol/L) did not significantly interfere on our AST, ALT, cholesterol, triglycerides, and urea, while its concentrations of 165.8 μmol/L started giving negative interference on uric acid. Turbidity (2+) did not interfere significantly on our AST and ALT but started giving positive interference on cholesterol, triglycerides, urea, and uric acid. Hemolysis (2+) gave positive interference on our cholesterol, triglycerides, urea, and uric acid. Stability of Ames Sera‐Pak working reagents was at least 30 days for AST, ALT, urea, and uric acid and 40 days for cholesterol and triglycerides. © 1992 Wiley‐Liss, Inc. Copyright © 1992 Wiley Periodicals, Inc., A Wiley CompanyMahidol UniversityBiochemistry, Genetics and Molecular BiologyHealth ProfessionsMedicineArticleSCOPUS10.1002/jcla.1860060502