Shigeru KitaniMitsuki YoshidaOusana BoonlucksanawongWatanalai PanbangredAtchareeya AnuegoonpipatTakeshi KurosuKazuyoshi IkutaYasuhiro IgarashiTakuya NihiraToyama Prefectural UniversityNational Institute of Infectious DiseasesOsaka UniversityMahidol UniversityNational Institutes of Health, Bethesda2019-08-282019-08-282018-07-01Journal of Antibiotics. Vol.71, No.7 (2018), 662-66618811469002188202-s2.0-85044324946https://repository.li.mahidol.ac.th/handle/20.500.14594/47309© 2018 The Author(s) under exclusive licence to the Japan Antibiotics Research Association. We identified a new cyclic lipodepsipeptide, cystargamide B (1), from the mycelial extract of a Kaempferia galanga rhizome-derived actinomycete strain, Streptomyces sp. PB013. The planar structure was elucidated based on high resolution fast-atom bombardment mass spectrometry (HRFABMS) spectroscopy and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopic data. The absolute configurations of the constituent amino acids were determined using advanced Marfey's method. Cystargamide B (1) includes rare structural units: a 5-hydroxytryptophan residue and a 2,3-epoxy fatty acid side chain. Notably, cystargamide B (1) inhibited the protease activity of the NS2B/NS3 complex from dengue virus.Mahidol UniversityPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1038/s41429-018-0044-0