Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor

Jiamvoraphong N.Nimsamer P.Wongsurawat T.Lorthongpanich C.Laowtammathron C.Issaragrisil S.Mahidol University2025-10-132025-10-132025-10-01Stem Cell Research Vol.88 (2025)18735061https://repository.li.mahidol.ac.th/handle/123456789/112555Pluripotent stem cell (PSC)-based technologies offer a promising new approach to generating functional cells or grafts for transplantation. To minimize the risk of immune rejection, induced pluripotent stem cells (iPSCs) that are homozygous for HLA Class I and Class II genes are a potential solution to reduce the risk of immune rejection in regenerative medicine applications. In this study, MUSIi023-A iPSC line was generated from peripheral blood mononuclear cells of a Thai donor with 11 loci homozygous HLA. This cell line is free of viral integration, exhibited pluripotent stem cell characteristics and able to differentiate to all embryonic lineages.Biochemistry, Genetics and Molecular BiologyMedicineEstablishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donorArticleSCOPUS10.1016/j.scr.2025.1038402-s2.0-1050179669981876775341033103