Wanchai AssavalapsakulDuncan R. SmithSakol PanyimMahidol University2018-07-242018-07-242003-08-04Diseases of Aquatic Organisms. Vol.55, No.3 (2003), 253-258017751032-s2.0-0042194959https://repository.li.mahidol.ac.th/handle/20.500.14594/20625Yellow head virus is a highly pathogenic agent that can cause a fatal disease in several species of penaeid shrimps. Using a primary cell culture and an in vitro quantal assay (TCID50), this study sought to determine the propagation profile of yellow head virus after inoculation at a low multiplicity of infection in the lymphoid tissue (oka organ) of Penaeus monodon. Detectable levels of virus were present as early as 24 h post-inoculation. Maximal viral yields were reached by 4 d post-infection, approximately 24 h after the onset of a detectable cytopathic effect, which was normally observable at 3 d post-inoculation. The methodology provides a useful tool for studying yellow head virus-host-cell interactions.Mahidol UniversityAgricultural and Biological SciencesPropagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodonArticleSCOPUS10.3354/dao055253