Isra IttaratH. Kyle WebsterYongyuth YuthavongMahidol UniversityArmed Forces Research Institute of Medical Sciences, Thailand2018-08-102018-08-101992-11-06Journal of Chromatography B: Biomedical Sciences and Applications. Vol.582, No.1-2 (1992), 57-64037843472-s2.0-0026455012https://repository.li.mahidol.ac.th/handle/20.500.14594/22252A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly. © 1992.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1016/0378-4347(92)80302-7