Auttawit SirichoatAroonlug LulitanondRattiyaporn KanlayaRatree TavichakorntrakoolAroonwadee ChanawongSujintana WongthongVisith ThongboonkerdKhon Kaen UniversityMahidol University2018-12-112019-03-142018-12-112019-03-142016-12-01Diagnostic Microbiology and Infectious Disease. Vol.86, No.4 (2016), 340-34418790070073288932-s2.0-84993977928https://repository.li.mahidol.ac.th/handle/20.500.14594/40957© 2016 Elsevier Inc. Reduced vancomycin susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem. Unfortunately, its genetic marker and molecular mechanisms remained unknown. This study investigated differential phenotypic characteristic and protein expression profiles among three groups of MRSA isolates, including vancomycin-susceptible S. aureus (VSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) (n = 7 isolates/group). Phenotypic characteristic revealed significant greater number of isolates with non-spreading colony in VISA as compared to both VSSA and hVISA groups. 2-DE followed by nanoLC-MS/MS analyses revealed increased glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in both hVISA and VISA, whereas 50S ribosomal protein L14 (RplN) and DNA-binding protein II (Hup) were increased only in VISA. The non-spreading colony and GAPDH level of MRSA may be used as the markers for differentiation of VSSA, hVISA and VISA.Mahidol UniversityMedicineArticleSCOPUS10.1016/j.diagmicrobio.2016.09.011