Chumpol PholpramoolMontri ChulavatnatolPawinee PiyachaturawatDamrongtheb Bunnag2024-07-252024-07-25198919892024Thesis (M.Sc. (Physiology))--Mahidol University, 1989https://repository.li.mahidol.ac.th/handle/20.500.14594/99815Physiology (Mahidol University 1989)The possible effects of some antifertility agents on the epididymal fluid and sperm surface proteins were investigated in adult male Fischer rats treated with sulfapyridine (SP), 450 mg/kg/day for 6 weeks, or alpha chlorohydrin (AC), 9 mg/kg/day for 1 week. At the end of treatment period, luminal fluid contents from the caput and cauda epididymidis were collected by micropuncture or retrograde perfusion of the vas deferens. Sperm concentration was counted in a haemocytometer chamber. Both epididymal fluid and sperm surface proteins were analyzed by linear gradient (7-15%) SDS-PAGE. Protein concentrations of the sperm free epididymal fluids and protein contents of the epididymal sperm extracts were determined by Lowrys method. SP and Ac produced a marked reduction infertility being 33.3±7.2 (n = 15) % and 14.0±7.0 (n = 7) % compared to their corresponding control values of 96.4±1.5 (n = 18) % and 94.0±2.8 (n=8)%, respectively. The concentrations of spermatozoa in both the caput and the cauda fluids were significantly decreased by SP or Ac treatment. In the caput fluids, the sperm concentrations after SP and AC treatments were, respectively. 0.13±0.02 (n=8) and 0.18±0.03 (n = 6) X10(9) cells/ml compared to their corresponding control values of 0.43±0.1(n=7) and 0.38±0.11(n=7) x 10(9) cells/ml, whereas in the cauda fluids the values after treatment were 0.96±0.15 (n=15)and 0.76±0.18(n=9) x 10(9) cells/ml compared to their corresponding control values of 1.77±0.25 (n=17) and 1.45±0.25(n=9)x10(9) cells/ml. respectively. However,there appears to be no changes in protein concentration of fluid and protein contents of sperm in the caput or cauda of the treated groups. On the other hand, SDS-PAGE revealed a reduction in intensity of some minor protein bands of the caput fluid with MWs of 32K, 31K, 28K, 26.5K and the cauda fluid with MWs 84K, 77k, 32K, 31K, 28k, 26.5K, 19K, 15.5K and 14.2K after both SP and AC treatments. However, SP and AC failed to cause any change in protein profiles of the caput sperm surface. Of particular interest was the increase of 3 additional bands with MWs of 85K, 44K and 28K from the cauda sperm extracts after SP treatment, whereas AC produce the reduction in intensity of proteins 50.5K and 49K, respectively. The results suggest that SP and AC may inhibit the fertilizing capacity of the rats spermatozoa directly by predicting an alteration of proteins on the cauda sperm surface and/or indirectly by changing the secretion or absorption processes of some proteins in both caput and cauda epididymal fluids.x, 120 leaves : ill.application/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าAlpha-ChlorohydrinEpididymis -- secretionFertility Agents, MaleProteinsSulfapyridineMaster ThesisMahidol University