Bungonsiri IntraJirayut EuanorasetrTakuya NihiraWatanalai PanbangredMahidol UniversityOsaka University2018-12-112019-03-142018-12-112019-03-142016-03-01Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-276014320614017575982-s2.0-84959162007https://repository.li.mahidol.ac.th/handle/20.500.14594/43090© 2015, Springer-Verlag Berlin Heidelberg. Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1007/s00253-015-7142-8