Gordon J. LeitchMahidol University2018-03-122018-03-121972-01-01Experimental and Molecular Pathology. Vol.17, No.2 (1972), 198-20510960945001448002-s2.0-0015414339https://repository.li.mahidol.ac.th/handle/123456789/10004A crude cholera toxin preparation, a purified cholera exoenterotoxin preparation and two commercial neuraminidase preparations were compared as to their effects on rabbit intestinal mucosa homogenate alkaline phosphatase and 5′-nucleotidase specific activity. Their effects on the brush border sucrase, trehalase, aryl amidase, and Mg-ATPase specific activities and sialic acids, phospholipids, cholesterol and glycolipids:protein ratios were also measured, together with their effects on microsome Mg-ATPase specific activity and sialic acids, phospholipids and cholesterol:protein ratios. The reduction in alkaline phosphatase specific activity seen with crude toxin was apparently due to the enterotoxin, as were the reductions in brush border sialic acids, and phospholipids:protein ratios and in microsome sialic acid: protein ratios. Commercial neuraminidase, at about 1000 times the activity found in the crude cholera toxin dose used, also reduced brush border and microsome sialic acids and lipids:protein ratios, but the pattern of the alterations in lipid:protein ratios differed from that seen with cholera enterotoxin. © 1972.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1016/0014-4800(72)90069-X