Zhong L.Lv X.Ling Z.Chaiwat W.Liu S.Zhang S.Lu X.Huda M.Huang Y.Cao J.P.Mahidol University2026-05-072026-05-072026-05-01Chinese Journal of Chemical Engineering Vol.93 (2026) , 9-1610049541https://repository.li.mahidol.ac.th/handle/123456789/116557The complex composition of bio-oil necessitates advanced separation strategies for its valorization. This work reports the application of biochar as a sustainable and structure-tunable stationary phase in column chromatography for the efficient fractionation of bio-oil. Biochars with distinctly different properties were engineered through pyrolysis of bamboo at 400 °C (bsC-400) and 700 °C (bsC-700). Comprehensive characterization confirmed that bsC-700 possessed a highly aromatized and hydrophobic surface with a well-developed porous network, while bsC-400 retained a polar and oxygen-functionalized surface. Their chromatographic performance revealed a fundamental structure-function relationship, as evidenced by the strong retention of sugars on the polar surface of bsC-400 via hydrogen-bonding interactions, which necessitated the use of aggressive solvents for elution. In contrast, bsC-700 exhibited exceptional affinity for phenolics via π-π stacking, while facilitating the early and efficient elution of sugars with moderately polar solvents due to its non-polar chemistry. This study establishes pyrolysis temperature as a critical design parameter for tailoring biochar selectivity, positioning it as a versatile stationary phase for targeting specific compound families within complex mixtures and advancing integrated biorefinery concepts.Chemical EngineeringChemistryEnvironmental ScienceBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.cjche.2025.12.0322-s2.0-105037366785