Chonticha SeekaSamran PrabpaiPalangpon KongsaereeSupinya TewtrakulThitima LhinhatrakoolSomyote SutthivaiyakitRamkhamhaeng UniversityMahidol UniversityPrince of Songkla UniversityRangsit University2018-12-212019-03-142018-12-212019-03-142017-06-23Journal of Natural Products. Vol.80, No.6 (2017), 1714-172415206025016338642-s2.0-85021128411https://repository.li.mahidol.ac.th/handle/20.500.14594/41867© 2017 The American Chemical Society and American Society of Pharmacognosy. Five 12,20-epoxypregnane glycosides (1-3, 5, and 6) and two 11,12-seco-pregnane glycosides (4 and 7) with spirodilactone motifs, as well as spirodilactone cleavage products 8 and 9, were isolated from the stems of Hoya kerrii. The relative configurations of the three related skeletons were supported by ROESY experiments and X-ray crystallographic analyses. The isolates were evaluated for their anti-inflammatory activity based on the inhibition of NO production in RAW264.7 cells, and some showed IC50values ranging from 12.6 to 96.5 μM. The most potent compound, 9a, was also examined for its anti-inflammatory mechanism against mRNA expression and was found to down-regulate mRNA expression of iNOS and COX-2 in a dose-dependent manner.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1021/acs.jnatprod.6b00730