Sarunporn TandhavanantGumphol WongsuvanVanaporn WuthiekanunNittaya TeerawattanasookNicholas P J DayDirek LimmathurotsakulSharon J. PeacockNarisara ChantratitaMahidol UniversitySappasitthiprasong HospitalNuffield Department of Clinical MedicineUniversity of Cambridge2018-10-192018-10-192013-07-01American Journal of Tropical Medicine and Hygiene. Vol.89, No.1 (2013), 165-168000296372-s2.0-84880620964https://repository.li.mahidol.ac.th/handle/20.500.14594/31903The diagnosis of melioidosis depends on the culture of Burkholderia pseudomallei, which takes at least 48 hours. We used a polyclonal-FITC-based immunofluorescence microscopic assay (Pab-IFA) on clinical samples to provide a rapid presumptive diagnosis. This has limitations including photobleaching and batch-to-batch variability. This study evaluated an IFA based on a monoclonal antibody specific to B. pseudomallei (Mab-IFA) and Alexa Fluor 488. A diagnostic evaluation was performed on a prospective cohort of 951 consecutive patients with suspected melioidosis. A total of 1,407 samples were tested. Test accuracy was defined against culture as the gold standard, and was also compared against Pab-IFA. A total of 88 samples from 64 patients were culture positive for B. pseudomallei. The diagnostic sensitivity and specificity of the Mab-IFA was comparable to the Pab-IFA (48.4% versus 45.3% for sensitivity, and 99.8% versus 98.8% for specificity). We have incorporated the Mab-IFA into our routine practice.© 2013 by The American Society of Tropical.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.13-0066