Karen L. LaurieOthmar G. EngelhardtJohn WoodAlan HeathJacqueline M. KatzMalik PeirisKatja HoschlerOlav HungnesWenqing ZhangMaria D. Van KerkhoveJaccqueline KatzXiuhua LuMin LevineVic VeguillaFeng LiuYaohui BaiPilaipan PuthavathanaHatairat LerdsamranPhisanu PoorukKnnika NateeromMaria Rita CastrucciIsabella DonatelliMarzia FacchiniNoriko KishidaMasato TashiroTakato OdagiriShailesh D. PawarSadhana S. KodeAnthony HawksworthRyan OrtiguerraGary BriceNicholas MartinTad KochelJose SanchezMichael CooperJames CummingsRalf WagnerConstanze GoepfertNina AlexJoanna HammannBritta NeumannMahendra PereraEmanuele MontomoliGuilia LapiniSara SbragiTian BaiZaijiang YuJianfang ZhouLouise CarolanKaren LaurieVictorian Infectious Diseases Reference LaboratoryNational Institute for Biological Standards and ControlCenters for Disease Control and PreventionThe University of Hong KongPublic Health EnglandNorwegian Institute of Public HealthOrganisation Mondiale de la SanteInstitut Pasteur, ParisMahidol UniversityIstituto Superiore Di SanitaNational Institute of Infectious DiseasesNational Institute of Virology IndiaU.S. Naval Health Research CenterNaval Medical Research CenterPaul-Ehrlich-InstitutUniversita degli Studi di SienaChinese National Influenza Center2018-11-232018-11-232015-01-01Clinical and Vaccine Immunology. Vol.22, No.8 (2015), 957-9641556679X155668112-s2.0-84939480223https://repository.li.mahidol.ac.th/handle/20.500.14594/35654Copyright © 2015, American Society for Microbiology. All Rights Reserved. The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1128/CVI.00278-15