Jiraphan JunjhonThomas J. EdwardsUtaiwan UtaipatValorie D. BowmanHeather A. HoldawayWei ZhangPoonsook KeelapangChunya PuttikhuntRushika PereraPaul R. ChipmanWatchara KasinrerkPrida MalasitRichard J. KuhnNopporn SittisombutChiang Mai UniversityPurdue UniversityThailand National Center for Genetic Engineering and BiotechnologyMahidol University2018-09-242018-09-242010-08-01Journal of Virology. Vol.84, No.16 (2010), 8253-8358109855140022538X2-s2.0-77954972691https://repository.li.mahidol.ac.th/handle/20.500.14594/29207During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage. Copyright © 2010, American Society for Microbiology. All Rights Reserved.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1128/JVI.00696-10