Pipop SaikaewJiale FuA. F.M.Almas ChowdhuryRicardo M. CarvalhoHidehiko SanoHokkaido UniversityMahidol UniversityThe University of British ColumbiaChina Medical University Shenyang2020-01-272020-01-272019-06-01Clinical Oral Investigations. Vol.23, No.6 (2019), 2629-263514363771143269812-s2.0-85055454577https://repository.li.mahidol.ac.th/handle/123456789/50717© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Objectives: To evaluate the effects of air-blowing time and storage time on microtensile bond strength (μTBS) of universal adhesives to dentin. Materials and methods: Ninety flat dentin surfaces from extracted human third molars were bonded with three universal adhesives (Clearfil Universal Bond-CU; G-Premio Bond-GP; Scotchbond Universal Adhesives-SB). Bonded dentin surfaces were air-dried for 5 s, 15 s, or 30 s followed by resin composite built-up. Resin-dentin beams were tested with μTBS test after different storage time in distilled water (24 h and 1 year). Data were analyzed by three-way ANOVA and Duncan test at (α = 0.05). Failure mode and resin-dentin interfaces were observed using a scanning electron microscope (SEM). Specific features of fractured beams after μTBS were further observed using SEM at high magnification. Results: Extension of air-blowing time from 5 s to 30 s increased the 24 h μTBS of CU only. Bond strength of all adhesives significantly decreased after 1-year storage except for CU at 5 s and 30 s of air-blowing time. One-year μTBS were significantly higher when air-blowing times were extended to 15 s for SB and 30 s for CU. Air-blowing time had no influence on GP. Conclusion: The effect of air-blowing time and storage time on resin-dentin bond was material-dependent. Clinical relevance: Extended air-blowing time increased the bond strength and bond durability of CU. Extension of air-blowing time to 15 s and 30 s improve the long-term bond strength of SB and CU, respectively.Mahidol UniversityDentistryArticleSCOPUS10.1007/s00784-018-2656-y