Phimonphan ChuankhayanYanling HuaJisnuson SvastiSanti SakdaratPatrick A. SullivanJames R. Ketudat CairnsSuranaree University of TechnologyMahidol UniversityMassey University2018-06-212018-06-212005-08-01Phytochemistry. Vol.66, No.16 (2005), 1880-1889003194222-s2.0-23944471715https://repository.li.mahidol.ac.th/handle/123456789/16204A β-glycosidase was purified from the seeds of Dalbergia nigescens Kurz based on its ability to hydrolyse p-nitrophenyl β-glucoside and β-fucoside. This enzyme did not hydrolyze various glycosidic substrates efficiently, so it was used to identify its own natural substrates. Two substrates were identified, isolated and their structures determined as: compound 1, dalpatein 7-O-β-d-apiofuranosyl-(1 → 6)-β-d- glucopyranoside and compound 2, 6,2′,4′,5′-tetramethoxy-7- hydroxy-7-O-β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranoside (dalnigrein7-O-β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranoside). The β-glycosidase removes the sugar from these glycosides as a disaccharide, despite its initial identification as a β-glucosidase and β-fucosidase. © 2005 Elsevier Ltd. All rights reserved.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.phytochem.2005.06.024