Chusuth P.Raksat P.Hannongbua S.Wangkanont K.Ounjai P.Rungrotmongkol T.Mahidol University2023-05-242023-05-242023-02-01ScienceAsia Vol.49 No.1 (2023) , 8-1415131874https://repository.li.mahidol.ac.th/handle/20.500.14594/82805Human α-7 nicotinic acetylcholine receptor (α-7 nAChR) is an ion channel that plays a significant function in neuronal communication and development. Defects of α-7 nAChR could cause plethora of neurologic and psychiatric impairments such as schizophrenia, Alzheimer’s, and inflammatory disorders. Hence, α-7 nAChR is an interesting candidate for drug development targeting neurological and inflammatory diseases. Therefore, the ability to obtain significant amount of the α-7 nAChR extracellular domain is crucial for further biochemical and structural investigations. We constructed a Pichia pastoris yeast strain that secretes the α-7 nAChR extracellular domain. The recombinant protein was hyperglycosylated and could be deglycosylated with Endoglycosidase H treatment. Cross-linking experiments suggested that the recombinant protein could form oligomeric states, consistent with the known property of the native α-7 nAChR. The optimized expression yield was 4.9 mg/l.MultidisciplinaryArticleSCOPUS10.2306/scienceasia1513-1874.2022.1102-s2.0-85152109017