Arada Rojana-udomsartChalermchai MitrpantChristine BundellLoren PriceYue Bei LuoVictoria FabianSteve D. WiltonPeter HollingsworthFrank L. MastagliaUniversity of Western AustraliaMahidol UniversityQueen Elizabeth II Medical Centre TrustRoyal Perth Hospital2018-10-192018-10-192013-09-16Journal of Neuroimmunology. Vol.264, No.1-2 (2013), 65-7018728421016557282-s2.0-84886773294https://repository.li.mahidol.ac.th/handle/20.500.14594/31869The mechanism of necrotizing myopathy associated with antibodies to signal recognition particle (SRP) remains unclear. We investigated the effect of anti-SRP. +. serum and complement on cell viability in myoblast cultures. Cell viability was only slightly reduced by incubation with anti-SRP. +. serum compared with control serum. However, the addition of fresh complement resulted in a marked reduction in cell survival. Surface immunostaining for SRP, C3c and C5b-9 was demonstrated in cultures pre-incubated with anti-SRP. +. serum and complement, and in muscle biopsies from patients with myopathy. These findings provide further support for a complement-dependent antibody-mediated mechanism in anti-SRP associated myopathy. © 2013 Elsevier B.V.Mahidol UniversityImmunology and MicrobiologyMedicineNeuroscienceArticleSCOPUS10.1016/j.jneuroim.2013.08.008