Adisak WongkajornsilpKhanit Sa-NgiamsuntornSuradej HongengMahidol University2018-06-112018-06-112012-01-20Methods in Molecular Biology. Vol.826, (2012), 73-87106437452-s2.0-84855910106https://repository.li.mahidol.ac.th/handle/20.500.14594/13821Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies. © 2012 Springer Science+Business Media, LLC.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1007/978-1-61779-468-1_7