Jintanat AnanworanichTanakorn ApornpongPope KosalaraksaTanyathip JaimulwongRawiwan HansudewechakulChitsanu PancharoenTorsak BunupuradahMom ChandaraThanyawee PuthanakitChaiwat NgampiyasakulJurai WongsawatSuparat KanjanavanitWicharn LuesomboonPhennapha KlangsinsirikulNicole Ngo-Giang-HuongStephen J. KerrSasiwimol UbolyamTawan MengthaisongRebecca S. GelmanKovit PattanapanyasatVonthanak SaphonnKiat RuxrungthamWilliam T. ShearerThe HIV Netherlands Australia Thailand Research CollaborationSouth East Asia Research Collaboration With HawaiiChulalongkorn UniversityKhon Kaen UniversityChiangrai Prachanukroh HospitalNational Institute of Public HealthPrapokklao HospitalBamrasnaradura Infectious Diseases InstituteNakornping HospitalQueen Savang Vadhana Memorial HospitalChiang Mai UniversityIRD U174-Program for HIV Prevention and TreatmentNational Center for HIV Epidemiology and Clinical ResearchHarvard School of Public HealthDana-Farber Cancer InstituteMahidol UniversityNational Center for HIV/AIDSBaylor College of Medicine2018-09-242018-09-242010-12-01Journal of Allergy and Clinical Immunology. Vol.126, No.6 (2010)009167492-s2.0-78649846344https://repository.li.mahidol.ac.th/handle/20.500.14594/29172Background: There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children. Objectives: To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls. Methods: We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4+T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children ≥8 years old with CD4+T-cell counts ≥350 cells/mm3, were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53). Results: Compared with controls, HIV-positive children had lower values (cell count per mm3and percent distribution) for THcells and higher values for cytotoxic T cells, with reductions in populations of naive THand cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated THand cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of THand cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated THand cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male. Conclusion: Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for THcells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated TH, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies. © 2010 American Academy of Allergy, Asthma & Immunology.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.jaci.2010.09.038