Srimachai T.Thipmunee M.Longsoh Y.Manaswin P.Phuket K.R.N.Srimachai T.Manaswin P.Phuket K.R.N.Thipmunee M.Longsoh Y.Srimachai T.Phuket K.R.N.Srimachai T.Phuket K.R.N.Srimachai T.Phuket K.R.N.Mahidol University2026-01-282026-01-282025-11-01Asean Journal of Scientific and Technological Reports Vol.28 No.6 (2025)https://repository.li.mahidol.ac.th/handle/123456789/114078The challenge posed by canned seafood wastewater (CSW) involves a low COD of 6.80 g/L and a high protein concentration of 3.56 g/L, making it unsuitable for hydrogen and methane production. Consequently, the potential return on investment for establishing a commercial system remains inadequate. To address this issue, a two-stage anaerobic digestion system incorporating co-digestion with glycerol waste (GW) was implemented. The two-stage co-digestion of CSW with GW, at various mixing ratios of 99:1, 98:2, 97:3, 96:4, and 95:5% (v/v), resulted in hydrogen yields of 15.6, 33.6, 38.7, 65.0, and 6.3 ml H<inf>2</inf>/g COD, respectively, while methane yields were measured at 311, 320, 326, 345, and 99 ml CH<inf>4</inf>/g COD, correspondingly. The ideal conditions for achieving the highest yields of hydrogen and methane from the anaerobic co-digestion of CSW with GW were found to be at a mixing ratio of 96:4% (v/v). The ongoing production of hydrogen and methane in a two-stage process utilizing CSTR-PFR and CSTR-CSTR reactors can yield hydrogen and methane at rates of 27.44 and 163.61 L/L of wastewater, and 20.41 and 145.35 L/L of wastewater, respectively. Anaerobic co-digestion of CSW with GW could enhance the production of hydrogen and methane from a two-stage anaerobic digestion system.Chemical EngineeringAgricultural and Biological SciencesArticleSCOPUS10.55164/ajstr.v28i6.2597432-s2.0-10502801728827738752