Pimolpan PithayanukulPakatip RuenraroengsakRapepol BavovadaNarumol PakmaneeRutt SuttisriMahidol UniversityChulalongkorn UniversityThai Red Cross Agency2018-08-242018-08-242007-02-01Pharmaceutical Biology. Vol.45, No.2 (2007), 94-9717445116138802092-s2.0-33947227397https://repository.li.mahidol.ac.th/handle/20.500.14594/24254The in vitro venom neutralizing capacity of tannic acid against the activities of Naja kaouthia (Naja naja kaouthia Lesson [Elapidae]) venom was investigated. Tannic acid was found to be effective in neutralizing the activities of Naja kaouthia (NK) venom. The lethal effect of four-times the LD50 in mice and the necrotizing effect of one minimum necrotizing dose (1 MND) in rats of NK venom were fully inhibited by tannic acid at ≥ 431 μg/mouse, with the median effective dose (ED50) of 334 μg/mouse and ≥ 30 μg/rat, respectively. The acetylcholinesterase activity of NK venom was almost completely neutralized (≥ 99%) by tannic acid at ≥ 1% w/v. It was evident that tannic acid was nontoxic and did not cause either a lethal effect in mice (at its maximum tested dose of 845 μg/mouse) or necrotic lesions in rats (at doses between 7.5 and 60 μg/rat). © 2007 Informa Healthcare.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1080/13880200601112885