Sangloung, C.Buddhirongawatr, R.Tantawiwattananon, N.Sukthana, Y.Charoonluk Sangloung.Nitipan Tantawiwattananon.Ruangrat Buddhirongawatr.Yaowalark Sukthana.Mahidol University. International College. Planning Research and Development Department.2014-10-272018-02-222014-10-272018-02-222014-10-272006Southeast Asian Journal Tropical Medicine Public Health. Vol.37 (Suppl. 3), (2006), 18-20https://repository.li.mahidol.ac.th/handle/20.500.14594/8779In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,00120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4°C before use.engMahidol University.Cryptosporidium culture.Animal model.Article