Haik ChosrowjanSeiji TaniguchiThanyaporn WongnateJeerus SucharitakulPimchai ChaiyenFumio TanakaInstitute for Laser TechnologyMahidol UniversityChulalongkorn University2018-06-112018-06-112012-04-15Journal of Photochemistry and Photobiology A: Chemistry. Vol.234, (2012), 44-48101060302-s2.0-84859543802https://repository.li.mahidol.ac.th/handle/20.500.14594/13920Ultrafast fluorescence dynamics of flavin adenine dinucleotide (FAD) in wild type pyranose 2-oxidase (P2O) has been investigated in solution by means of fluorescence up-conversion method. Fluorescence decays were well described by two-exponential model function. Fluorescence lifetimes were τ 1 ∼ 110 fs and τ 2 ∼ 360 ps, respectively. The ( τ2 / τ1 ) ratio (∼3200) was extraordinary high compared to other flavoproteins without subunit structure. The heterogeneous distribution of emission lifetimes were elucidated in terms of two different conformers of P2O; conformer 1 with τ 1 and conformer 2 with τ 2 . Emission peaks of conformer 1 and conformer 2 were determined to be at ∼540 nm and 510 nm, respectively, using transient spectral reconstruction procedure. Using dynamics analysis by Kakitani and Mataga (KM) theory, both quenching processes were ascribed to photoinduced electron transfer (ET) reactions mainly from Trp168 to the excited isoalloxazine (Iso*) in different protein tetramers having different static dielectric constants (ε 1 ∼ 3.25 for conformer 1 and ε 2 ∼ 5.93 for conformer 2). The quaternary structure seems to be responsible for the observed conformational heterogeneity. © 2012 Elsevier B.V. All rights reserved.Mahidol UniversityChemical EngineeringChemistryPhysics and AstronomyArticleSCOPUS10.1016/j.jphotochem.2011.11.013