Savaspun K.Thepmalee C.Myo H.Arinno A.Phanumartwiwath A.Aongbangkhen C.Eurtivong C.Boonchaisri S.Ninjiaranai P.Sam-Ang P.Mahidol University2024-11-152024-11-152024-09-01Records of Natural Products Vol.18 No.5 (2024) , 514-51913076167https://repository.li.mahidol.ac.th/handle/20.500.14594/102026A new naturally occurring diarylheptanoid, (1E,4Z,6E)-5-hydroxy-1,7-diphenylhepta-1,4,6-trien-3-one (3), was isolated from the rhizomes of K. elegans along with six known compounds, flavokawain B (1), 5,6-dehydrokawain (2), pinocembrin (4), cardamonin (5), alpinetin (6), and crotepoxide (7), among which compound 6 had not previously been isolated from this plant species. Two chalcones, flavokawain B (1) and cardamonin (5) were active against nitric oxide (NO) radicals released from LPS-induced RAW264.7 macrophages, resulting in 91.58% and 98.68% inhibition of NO production, respectively. Furthermore, compounds 1 and 5 showed superior cytotoxicity against MCF-7 (IC50 = 23.07 and 20.84 µM) and MDA-MB-231 (IC50 = 21.77 and 26.64 µM) cell lines, respectively. In silico molecular modeling studies of the most active compounds 1 and 5 against epidermal growth factor receptors (EGFR) suggested that π–π interactions with residues on the EGFR protein contributed to their anticancer properties. The results suggest that cardamonin (5) could be a promising candidate for further development of anti-inflammatory and anticancer agents.Pharmacology, Toxicology and PharmaceuticsChemistryAgricultural and Biological SciencesArticleSCOPUS10.25135/rnp.472.2406.32502-s2.0-85208435129