Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi

Mallika ImwongNaowarat TanomsingSasithon PukrittayakameeNicholas P J DayNicholas J. WhiteGeorges SnounouMahidol UniversityRoyal InstituteChurchill HospitalHopital Pitie SalpetriereSorbonne UniversiteNational University of Singapore, Faculty of Medicine2018-09-132018-09-132009-12-01Journal of Clinical Microbiology. Vol.47, No.12 (2009), 4173-4175009511372-s2.0-71549116085https://repository.li.mahidol.ac.th/handle/20.500.14594/27858The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes. Copyright © 2009, American Society for Microbiology. All Rights Reserved.Mahidol UniversityMedicineSpurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesiArticleSCOPUS10.1128/JCM.00811-09