Khomsan TiensomjitrRattha NooratSinchai ChomngamKanokorn WechakornSamran PrabpaiPhongthon KanjanasiriratYongyut PewkliangSuparerk BorwornpinyoPalangpon KongsaereeMahidol University2019-08-232019-08-232018-04-15Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. Vol.195, (2018), 136-141138614252-s2.0-85041475475https://repository.li.mahidol.ac.th/handle/20.500.14594/45498© 2018 Elsevier B.V. A rhodol-based fluorescent probe has been developed as a selective hydrazine chemosensor using levulinate as a recognition site. The rhodol levulinate probe (RL) demonstrated high selectivity and sensitivity toward hydrazine among other molecules. The chromogenic response of RL solution to hydrazine from colorless to pink could be readily observed by the naked eye, while strong fluorescence emission could be monitored upon excitation at 525 nm. The detection process occurred via a ring-opening process of the spirolactone initiated by hydrazinolysis, triggering the fluorescence emission with a 53-fold enhancement. The probe rapidly reacted with hydrazine in aqueous medium with the detection limit of 26 nM (0.83 ppb), lower than the threshold limit value (TLV) of 10 ppb suggested by the U.S. Environmental Protection Agency. Furthermore, RL-impregnated paper strips could detect hydrazine vapor. For biological applicability of RL, its membrane-permeable property led to bioimaging of hydrazine in live HepG2 cells by confocal fluorescence microscopy.Mahidol UniversityChemistryPhysics and AstronomyArticleSCOPUS10.1016/j.saa.2018.01.033