Uday Younis Hussein AbdullahHishamshah M. IbrahimHaitham Muhammed JassimMohamad Zaki SallehTeh Lay KekMohd Nur Fakhruzzaman bin NoorizhabBin Alwi ZilfalilPrapin WilairatSuthat FucharoenUniversiti Sultan Zainal AbidinKuala Lumpur HospitalMahidol UniversityUniversiti Teknologi MARASchool of Medical Sciences - Universiti Sains MalaysiaSouth Metropolitan Health Service2020-01-272020-01-272019-07-01Biochemistry and Biophysics Reports. Vol.18, (2019)240558082-s2.0-85064628676https://repository.li.mahidol.ac.th/handle/123456789/50144© 2019 This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (β-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of β-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different β-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with β-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the β-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.bbrep.2019.100635