Harper R.Ley B.Kabir M.A.Matulis G.von Seidlein L.Alam M.S.Adhikari B.Okech B.A.Williams A.L.Price R.N.von Fricken M.E.Mahidol University2024-05-222024-05-222024-01-01PloS one Vol.19 No.5 (2024) , e0297918https://repository.li.mahidol.ac.th/handle/123456789/98426Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.MultidisciplinaryArticleSCOPUS10.1371/journal.pone.02979182-s2.0-851929126151932620338728310