Sasitorn RungarunlertJoao N. FerreiraAndras DinnyesMahidol UniversityNational University of Singapore, Faculty of DentistryBioTalentum Ltd.Szent Istvan Egyetem2018-12-112019-03-142018-12-112019-03-142016-01-01Methods in Molecular Biology. Vol.1502, (2016), 169-179106437452-s2.0-84990230657https://repository.li.mahidol.ac.th/handle/123456789/43200© Springer Science+Business Media New York 2016. Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactorbased methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChapterSCOPUS10.1007/7651_2016_341