Prayad KomaratatPrapon WilairatPichit TosukhowongChaveewon Porntadavity2025-02-032025-02-03198820251988Thesis (M.Sc. (Biochemistry))--Mahidol University, 1988https://repository.li.mahidol.ac.th/handle/20.500.14594/103394Biochemistry (Mahidol University 1988)The purpose of this study was to find out the mechanism for the hypocholesterolemic effect of vitamin E in rabbits. The animals were fed a purified basal diet deficient in vitamin E and were supplemented with varying amounts of DL-?-tocopherol acetate, namely 21, 210 and 2,100 mg/week for 10 weeks. There was a significant increase in plasma cholesterol in rabbits supplemented with only corn oil after 6 weeks. Increased vitamin E supplementation reduced plasma cholesterol level. In liver, cholesterol and phospholipid contents were not significantly different in rabbits supplemented with varying amount of vitamin E. The activity of HMG-CoA reductase was also not affected by high level of vitamin E. Liver membranes prepared from rabbits supplemented with these amounts of vitamin E showed no differences in total binding with (125)I-LDL, indicating that the number of LDL receptors did not increase as the amount of vitamin E supplementation increased. The results of in vitro binding were consistent with the finding that the clearance rates of (125)I-LDL in rabbits supplemented with varying amounts of vitamin E were not significantly different. These results suggested that decreased plasma cholesterol in vitamin E supplemented rabbits did not involve cholesterols metabolism in liver. The decreased plasma cholesterol in vitamin E supplemented rabbits may be due to a decrease in absorption of cholesterol.viii, 94 leaves : ill. 30 cmapplication/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าAnticholesteremic AgentsCholesterol-metabolismVitamin ERabbitsMaster ThesisMahidol University