Sakchai ChaibunWilart PompimonChanika TidchaiNoraset ChalaemwongwanJutarut WongpingChidawan KansornPhansuang UdomputtimekakulPuttinan MeepowpanSiriporn JungsuttiwongSarinya HadsadeeSuttiporn PikulthongNarong NuntasaenThailand Ministry of Natural Resources and EnvironmentUbon Ratchathani UniversityMahidol UniversityChiang Mai UniversityLampang Ra-jabhat University2022-08-042022-08-042021-01-01Natural Products Journal. Vol.11, No.4 (2021), 559-56822103163221031552-s2.0-85120606386https://repository.li.mahidol.ac.th/handle/20.500.14594/78550Background: C. delpyi, C. decalvatus and C. caudatus are in the Euphorbiaceae family. The aerial parts, such as twigs, leaves and barks of these plants are used as traditional medicine such as anti-inflammatory, cytotoxicity, and antifungal properties. Objectives: The aims of this work were 1) to study the chemical composition of C. delpyi, C. decal-vatus, and C. caudatus 2) to test the antibacterial, anti-HIV-1 RT, and cytotoxicity activities of crude extracts and pure compounds from these plants. Methods: Extraction, separation and purification of three plants were performed following chroma-tographic method. The biological activities, including antibacterial, anti-HIV-1 RT and cytotoxicity assay of three plants, were evaluated by the standard methods. Results: Phytochemical investigation of C. delpyi highlighted a new clerodane diterpenoid; cro-tondelpyitin A (1). The five known compounds were found, such as acetyl aleuritolic acid (2), 5-hydroxy-7,4′-dimethoxyflavone (3), and pilloin (4) in C. decalvatus and 3α-benzoyloxy-D while A-friedo-oleanan-27,16α-lactone (5), and bergenin (6) in C. caudatus. Compound 3 showed the most effective antibacterial activities with MIC in range <0.16-1.25 mg/mL, and MBC in range 0.6->5.0 mg/mL. The six compounds were inactive with anti-HIV-1 RT. In addition, compound 4 was active for cytotoxic activities on FaDu and KKU-M213 at <4 µg/mL. Conclusion: The present study reveals that the Croton species are sources of diterpenoid-type compounds and a significant guide for further research of chemical constituents from these plants as potential medicines.Mahidol UniversityMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.2174/2210315510999200704143606