Wansadaj JaroenramNarong ArunrutWansika KiatpathomchaiMahidol UniversityJames Cook University, AustraliaThailand National Center for Genetic Engineering and Biotechnology2018-06-112018-06-112012-11-19Journal of Virological Methods. Vol.186, No.1-2 (2012), 36-4218790984016609342-s2.0-84869067382https://repository.li.mahidol.ac.th/handle/20.500.14594/14242Salt-induced self-aggregation of gold nanoparticles (AuNP) carrying unisense ssDNA probes can be prevented specifically by complementary DNA. Loop-mediated isothermal amplification (LAMP) can amplify DNA rapidly. Here, the two techniques were combined to detect yellow head virus (YHV). The LAMP-AuNP method required 60. min for LAMP and 5. min for hybridization of LAMP products to an AuNP-labeled ssDNA probe followed by salt induced probe-particle aggregation to visualize color development. The detection sensitivity of the method was comparable to that of the commercial IQ2000™ nested RT-PCR but only required ∼65. min to produce a result, and did not cross-detect other shrimp viruses. As the LAMP-AuNP protocol only requires a heating block, it offers opportunities for rapid detection of YHV. © 2012 Elsevier B.V.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/j.jviromet.2012.08.013