Sirasa YodmongkolBoonsong SutapunVerayuth PraphanphojToemsak SrikhirinThomas BrandstetterJürgen RüheMahidol UniversitySuranaree University of TechnologyRajanukul InstituteUniversität Freiburg im BreisgauMedical Genetics Center2018-12-112019-03-142018-12-112019-03-142016-03-01Sensing and Bio-Sensing Research. Vol.7, (2016), 95-99221418042-s2.0-85000365358https://repository.li.mahidol.ac.th/handle/20.500.14594/43109© 2016 The Authors. In this study, protein microarrays based on sandwich immunoassays are generated to quantify the amount of alpha fetoprotein (AFP) in blood serum. For chip generation a mixture of capture antibody and a photoactive copolymer consisting of N,N-dimethylacrylamide (DMAA), methacryloyloxy benzophenone (MaBP), and Na-4-styrenesulfonate (SSNa) was spotted onto unmodified polymethyl methacrylate (PMMA) substrates. Subsequently to printing of the microarray, the polymer and protein were photochemically cross-linked and the forming, biofunctionalized hydrogels simultaneously bound to the chip surface by short UV- irradiation. The obtained biochip was incubated with AFP antigen, followed by biotinylated AFP antibody and streptavidin-Cy5 and the fluorescence signal read-out. The developed microarray biochip covers the range of AFP in serum samples such as maternal serum in the range of 5 and 100 ng/ml. The chip production process is based on a fast and simple immobilization process, which can be applied to conventional plastic surfaces. Therefore, this protein microarray production process is a promising method to fabricate biochips for AFP screening processes.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyComputer ScienceEngineeringMaterials ScienceArticleSCOPUS10.1016/j.sbsr.2016.01.010