Pewpan M. IntapanTongjit ThanchomnangViraphong LulitanondPhunthira PongsaskulchotiWanchai MaleewongKhon Kaen UniversityMahidol University2018-09-132018-09-132009-11-01American Journal of Tropical Medicine and Hygiene. Vol.81, No.5 (2009), 917-920000296372-s2.0-70449701844https://repository.li.mahidol.ac.th/handle/20.500.14594/27637Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.2009.09-0275