Suchada SaoviengJinfu WuWei Horng JeanChih Yang HuangMatthew F. HigginsAhmad AlkhatibMallikarjuna KoriviChiao Nan ChenChia Hua KuoTzu Chi University of Science and TechnologyUniversity of TaipeiNational Yang-Ming University TaiwanFar Eastern Memorial Hospital TaiwanChina Medical University Hospital TaichungTeesside UniversityBuddhist Tzu-Chi General Hospital TaiwanTzu Chi UniversityUniversity of DerbyMahidol UniversityChina Medical University TaichungSouth China Normal UniversityZhejiang Normal University2020-05-052020-05-052020-01-01Cancer Medicine. (2020)204576342-s2.0-85082965466https://repository.li.mahidol.ac.th/handle/20.500.14594/54495© 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. Naturally occurring tumor in animals receiving high minerals from deep oceans (DOM: hardness 600 mg/L) from 6 months of age until natural death was firstly assessed in 200 Sprague Dawley rats, randomized into four groups: Control (C), DOM (D), Fructose (F), and Fructose + DOM (FD). Fructose drink contained 11% fructose. Tumor incidence (necropsy at death) in the D group was ~40% lower than that in the C group (P <.05), together with lower body mass gain and greater locomotive activity during their initial 18 months (P <.05) but not during later life. X-ray image analysis on abnormal solid tissue among survivors at 18 and 24 months of age confirms a similar trend, exhibiting ~50% and ~65% lower tumor incidence than the C and F groups, respectively. Reduced-to-oxidized glutathione ratio (GSH/GSSG) declined with age for the first three quarters of life on all groups (P <.05), followed by a resurgence during end-life among survivors at 24 months. This resurgence is markedly associated with lower tumor expansion but unrelated with DOM supplementation. Our results demonstrate valuable application of minerals and trace elements from deep oceans, as a vastly available natural source, on tumor suppression during normal aging.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1002/cam4.3028