Maliwan AmatatongchaiWongduan SroyseeSanoe ChairamDuangjai NacaprichaUbon Rajathanee UniversityFlow Innovation-Research for Science and Technology Laboratories (FIRST Labs.Mahidol University2018-12-212019-03-142018-12-212019-03-142017-05-01Talanta. Vol.166, (2017), 420-427003991402-s2.0-84949646249https://repository.li.mahidol.ac.th/handle/20.500.14594/41875© 2015 Elsevier B.V. We report a novel amperometric glucose biosensor based on glucose oxidase (GOx) immobilized on a carbon nanotube (CNTs)–poly(diallyldimethyl-ammonium chloride) (PDDA)–platinum nanoparticle (PtNPs) modified carbon-paste electrode (CNTs–PDDA–PtNPs/CPE). The CNTs–PDDA–PtNPs composite materials were characterized by TEM and electrochemical techniques. Cyclic voltammetric results reveal direct electron transfer of the immobilized GOx, indicated by two quasi-reversible redox peaks at a potential of 0.37 V (vs. Ag/AgCl) in phosphate buffered solution (PBS) (0.10 M, pH 7). The biosensor provides good glucose oxidation activity and retention of GOx electrocatalytic activity due to CNTs–PDDA–PtNPs enhancement of the redox response. The carbon paste electrode was installed as working electrode in a flow through electrochemical cell of a flow injection (FI) system. Glucose was quantified using amperometric measurements at 0.5 V vs. Ag/AgCl and PBS carrier (0.10 M, pH 7.0) at a flow rate of 1.0 mL min−1. The linear working ranges for glucose measurements were 0.1–3 mM (r2=0.995) and 5–100 mM (r2=0.997), with corresponding sensitivities of 0.127 and 0.060 (μA s) mM−1, respectively. The system provides good precision of 2.8% R.S.D with a calculated detection limit (3 S/N) of 15 μM. The proposed method was successfully applied to determination of glucose in food and pharmaceutical samples with throughput of 200 samples h−1.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1016/j.talanta.2015.11.072