Kowaboot S.Mahidol University2023-06-182023-06-182022-01-01Japanese Journal of Infectious Diseases Vol.75 No.1 (2022) , 24-3013446304https://repository.li.mahidol.ac.th/handle/20.500.14594/86683Mouse antibodies specific to dengue NS1 have been widely investigated for their crossreactivity with several human biomolecules. This is the first study demonstrating the cross-reactivity of human monoclonal antibodies (HuMAbs) specific to dengue NS1 isolated from patients infected with dengue virus serotype-2 (DENV-2). Nine anti-NS1 HuMAbs, which were mainly derived from patients in convalescent-phase after secondary infection of DENV-2, were characterized. Their cross-reactivity with plasminogen, thrombin, and endothelial cells was investigated, following which plasmin-formation assays were performed. All anti-NS1 HuMAbs exhibited cross-reactivity with human plasminogen (Plg), but not with thrombin or endothelial cells. Moreover, all HuMAbs exhibiting cross-reactivity with Plg converted Plg to plasmin in the plasmin-formation assay. These results suggest the implications and drawbacks of using anti-NS1 antibodies in immunotherapy.MedicineArticleSCOPUS10.7883/yoken.JJID.2020.10712-s2.0-851238138871884283634053951