Sarawut JitrapakdeeNathida PetchamphaiPiyanate SunyakumthornJohn C. WallaceVichai BoonsaengMahidol UniversityUniversity of Adelaide2018-09-072018-09-072001-09-21Biochemical and Biophysical Research Communications. Vol.287, No.2 (2001), 411-4170006291X2-s2.0-0035929365https://repository.li.mahidol.ac.th/handle/123456789/26444We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC 6.4.1.1]. The coding region contains 19 exons, one 5′-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5′-nested deletions of the 5′-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5′-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5′-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. © 2001 Academic Press.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1006/bbrc.2001.5599