Suparak ParaPunchalee MungkalasutMakamas ChandaIssarang NuchprayoonSrivicha KrudsoodChalisa Louicharoen CheepsunthornChulalongkorn UniversityMahidol University2019-08-282019-08-282018-01-01Mediterranean Journal of Hematology and Infectious Diseases. Vol.10, No.1 (2018)203530062-s2.0-85045118984https://repository.li.mahidol.ac.th/handle/123456789/47078© Mediterranean Journal of Hematology and Infectious Diseases 2018. Background: The protective effect of a-thalassemia, a common hematological disorder in Southeast Asia, against Plasmodium falciparum malaria has been well established. However, there is much less understanding of the effect of a-thalassemia against P. vivax. Here, we aimed to investigate the proportion of a-thalassemia including the impact of a-thalassemia and HbE on the parasitemia of P. vivax in Southeast Asian malaria patients in Thailand. Methods: A total of 210 malaria patients, admitted to the Hospital for Tropical Diseases, Thailand during 2011-2012, consisting of 159 Myanmeses, 13 Karens, 26 Thais, 3 Mons, 3 Laotians, and 6 Cambodians were recruited. Plasmodium spp. and parasite densities were determined. Group of deletion mutation (--SEA, -α3.7, -α4.2deletion) and substitution mutation (HbCS and HbE) were genotyped using multiplex gap-PCR and PCR-RFLP, respectively. Results: In our malaria patients, 17/210 homozygous and 74/210 heterozygous -α3.7 deletion were found. Only 3/210 heterozygous -α4.2 and 2/210 heterozygous--SEA deletion were detected. HbE is frequently found with 6/210 homozygotes and 35/210 heterozygotes. The most common thalassemia allele frequencies in Myanmar population were -α3.7 deletion (0.282), followed by HbE (0.101), HbCS (0.013), -α4.2 deletion (0.009), and --SEA deletion (0.003). Only density of P. vivax in a-thalassemia trait patients (-α3.7/-α3.7, --SEA/αα, -α3.7/-α4.2) but not in silent a-thalassemia (-α3.7/αα, -α4.2/αα, ααCS/αα) were significantly higher compared with non-a-thalassemia patients (p=0.027). HbE did not affect P. vivax parasitemia. The density of P. falciparum significantly increased in heterozygous HbE patients (p=0.046). Conclusions: Alpha-thalassemia trait is associated with high levels of P. vivax parasitemia in malaria patients in Southeast Asia.Mahidol UniversityMedicineArticleSCOPUS10.4084/mjhid.2018.015