Kasama SupanaranondSaowakon SukarayodhinManu TanyakasetKruavon BalachandraDuangrat JullaksornMati RienkijkarnNarin HoisankaSurang TantivanichThailand Ministry of Public HealthMahidol University2018-07-042018-07-041997-09-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.3 (1997), 631-640012515622-s2.0-0031219588https://repository.li.mahidol.ac.th/handle/123456789/18101This study was carried out to investigate the amplification of HER-2/neu oncogene in 66 patients with primary breast cancer and 90 samples from benign breast disease (BBD). The amplification of HER-2/neu oncogene in the DNA of paraffin-embedded specimens was determined by differential PCR. Nineteen out of 66 (28.8%) breast cancer patients showed amplification of the gene. No gene amplification was found in benign breast disease. There was no significant correlation of HER-2/neu amplification with, age, menopausal status, the number of positive nodes, tumor size, estrogen receptor, however, amplification of HER-2/neu gene was strongly correlated with nodal status (p = 0.0049). In node positive patients, the incidence of HER-2/neu amplification was high (43%). These findings indicate that the amplification of HER-2/neu gene may be of pathogenetic significance in breast cancer and may have a poor prognosis in node positive breast cancer patients while no gene amplification in benign breast disease suggests that HER-2/ neu amplification is a late molecular alteration event in the pathogenesis of breast cancer.Mahidol UniversityMedicineArticleSCOPUS